Whole Genome Assembly (v4.0) and Annotation of Zea mays - cv. B73 (EnsemblPlant/Gramene)

The gene models of Maize RefGen_V4 were named following the standard of Maize Genetics Nomenclature.

Genes were annotated with Maker pipeline (Campbell et al. 2014) using 111,000 transcripts obtained by single-molecule sequencing. These long read Iso-Seq data (Wang et al. 2016) improved annotation of alternative splicing, more than doubling the number of alternative transcripts from 1.5 to 3.8 per gene, thereby improving our knowledge of gene structure and transcript variation, resulting in substantial improvements including resolved gaps and misassembles, corrections to strand, consolidation of gene models, and anchoring of unanchored genes.

Source : Gramene